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Introduction: We hypothesize that equine pooled cryopreserved umbilical cord blood (eCB) MSC are safe and effective in treating surgically created equine osteoarthritis.
Material and Methods: Osteoarthritis (OA) was surgically induced in 6 Standardbreds using a fetlock chip model. Horses were exercised throughout the study. Horses were randomly selected to be treated with 10 Mio cryopreserved pooled intraarticular eCB-MSCs in HA (n = 3) or saline (n=3). Prior to fetlock chip creation, at various time points postoperatively and post treatment, lameness evaluations, radiographs, ultrasound, MRI and synovial fluid samples were taken. Semiquantitative scores of all modalities were obtained and compared between groups.
Results: Six weeks after fetlock chip creation the lameness score in the MSC group was 2.6 (range 2-3/5) and the control group 2.8 (range 2-3/5) and after treatment in the MSC group 1.5 (range 1-2) and in the control group 2.7 (range 1.5 – 3.5). Six weeks after treatment the median radiographic score of 5/39 (range 3-7/39) was lower in the MSC group than 9/39 (range 7-11/39) in the control group. 5 weeks after treatment the median WORMS score was lower with 18/154 (range 17 -20/154) after MSC treatment then after saline treatment with 27/154 (range 25-30/154). Six weeks after treatment the arthroscopic score was significantly lower in the MSC group after treatment (16.6/43 [13-21]) compared to the control group (21.6/43 [range 21-23]) (p=0.04).
Conclusion: Pooled cryopreserved allogeneic eCB MSC are safe to be used for intraarticular treatment and effective in reducing cartilage degeneration in osteoarthritis in horses.
33rd Annual Scientific Meeting proceedings
Stream:
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Session:
Date/Time: 30-11--0001 (00:00 - 00:00) | Location:
Date/Time: 30-11--0001 (00:00 - 00:00) | Location:
Mesenchymal stromal cells for the treatment of surgically induced equine fetlock osteoarthritis
Munevar Luque R1, Koch TG1, Goodrich L*2, Giraldo A1, Valverde A1, Zur Linden A1, Beehler Marfisi J1, Russel K1, Koenig J*1
1Ontario Veterinary College, University of Guelph, Guelph, Canada, 2Translational Medical Institute, Colorado State University, Fort Collins, USA.
Introduction: We hypothesize that equine pooled cryopreserved umbilical cord blood (eCB) MSC are safe and effective in treating surgically created equine osteoarthritis.
Material and Methods: Osteoarthritis (OA) was surgically induced in 6 Standardbreds using a fetlock chip model. Horses were exercised throughout the study. Horses were randomly selected to be treated with 10 Mio cryopreserved pooled intraarticular eCB-MSCs in HA (n = 3) or saline (n=3). Prior to fetlock chip creation, at various time points postoperatively and post treatment, lameness evaluations, radiographs, ultrasound, MRI and synovial fluid samples were taken. Semiquantitative scores of all modalities were obtained and compared between groups.
Results: Six weeks after fetlock chip creation the lameness score in the MSC group was 2.6 (range 2-3/5) and the control group 2.8 (range 2-3/5) and after treatment in the MSC group 1.5 (range 1-2) and in the control group 2.7 (range 1.5 – 3.5). Six weeks after treatment the median radiographic score of 5/39 (range 3-7/39) was lower in the MSC group than 9/39 (range 7-11/39) in the control group. 5 weeks after treatment the median WORMS score was lower with 18/154 (range 17 -20/154) after MSC treatment then after saline treatment with 27/154 (range 25-30/154). Six weeks after treatment the arthroscopic score was significantly lower in the MSC group after treatment (16.6/43 [13-21]) compared to the control group (21.6/43 [range 21-23]) (p=0.04).
Conclusion: Pooled cryopreserved allogeneic eCB MSC are safe to be used for intraarticular treatment and effective in reducing cartilage degeneration in osteoarthritis in horses.
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